Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.147053 |
Chromosome: | chromosome_5 |
Location: | 2951836 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g238280 | FAP205 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGGCCGCGGCTGACGATAGCGATGCCCCC |
Internal bar code: | TCGGTGGCAACGGGGCGGAGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 673 |
LEAP-Seq percent confirming: | 99.7347 |
LEAP-Seq n confirming: | 3384 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATGTACTGCCCCTTCTCGT |
Suggested primer 2: | ACGCACATGGTGGTCAGTAA |