| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147071 |
| Chromosome: | chromosome 2 |
| Location: | 1127108 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g081400 | BCA1 | Branched chain amino acid aminotransferase; (1 of 1) 2.6.1.88 - Methionine transaminase / Methionine-oxo-acid transaminase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCGTGCTGTAGCCGTACGACAGCAGGC |
| Internal bar code: | CGTTGGCCGTCAGCAAGGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 98.7307 |
| LEAP-Seq n confirming: | 2878 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTTTCCAACTGCTCAAA |
| Suggested primer 2: | CACCATCACTGTTTGGCAAC |