| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147096 |
| Chromosome: | chromosome 12 |
| Location: | 2813930 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502600 | SLT1 | Sodium/sulfate co-transporter; (1 of 2) PF00939//PF02080 - Sodium:sulfate symporter transmembrane region (Na_sulph_symp) // TrkA-C domain (TrkA_C) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAATTAATGCCCGCGTAATTAGCGCCAA |
| Internal bar code: | TTCATTTTAACGTATGCACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 880 |
| LEAP-Seq percent confirming: | 99.7262 |
| LEAP-Seq n confirming: | 6555 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATATATTCCCCCGAGGAT |
| Suggested primer 2: | CGAGAAAAAGGAACTGCTGG |