Insertion junction: LMJ.RY0402.147096_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.147096
Chromosome:chromosome 12
Location:2813933
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g502600 SLT1 Sodium/sulfate co-transporter; (1 of 2) PF00939//PF02080 - Sodium:sulfate symporter transmembrane region (Na_sulph_symp) // TrkA-C domain (TrkA_C) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCGCGGGGCTGCCCGTCAACCCCCCGTT
Internal bar code:CCTGTCCGTGGGCGAGCGTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:793
LEAP-Seq percent confirming:99.7339
LEAP-Seq n confirming:8619
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:GCATATATTCCCCCGAGGAT
Suggested primer 2:CGAGAAAAAGGAACTGCTGG