Insertion junction: LMJ.RY0402.147105_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.147105
Chromosome:chromosome_12
Location:8414242
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g548650 BBS4 Bardet Biedl syndrome-4 associated protein antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CCTGATGCGGAGCTGTGGGCACGACGCTGG
Internal bar code:AGAGCTGGCTCATATTGGCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:146
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGTACTGCATGCGAGAGACC
Suggested primer 2:GTCGGAGCGAAGAAACAGTC