| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147117 |
| Chromosome: | chromosome 5 |
| Location: | 2185848 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234649 | (1 of 13) PF00646 - F-box domain (F-box) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTAGCCAGCCCTCGCAAGGAAGTTCCTC |
| Internal bar code: | AAGGCGCTGCAACTGAATCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 198 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGTGTGCAGTGTATTCC |
| Suggested primer 2: | GATTGGGTGAGGGATAGGGT |