Insertion junction: LMJ.RY0402.147125_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.147125
Chromosome:chromosome 10
Location:1668001
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g429880 CGL156 DNA binding protein; (1 of 1) IPR001606//IPR001965//IPR003754//IPR011011//IPR019787 - ARID DNA-binding domain // Zinc finger, PHD-type // Tetrapyrrole biosynthesis, uroporphyrinogen III synthase // Zinc finger, FYVE/PHD-type // Zinc finger, PHD-finger CDS|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGGCGCCGGCGCGGCCGGAAATGCATGG
Internal bar code:CCTCCGCGCTAACGAGGCAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:251
LEAP-Seq percent confirming:73.2429
LEAP-Seq n confirming:1511
LEAP-Seq n nonconfirming:552
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCACATGTATGGATTCAG
Suggested primer 2:GCAGTCAAGAGTCCAGAGGG