| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147134 |
| Chromosome: | chromosome 13 |
| Location: | 4387374 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602950 | (1 of 1) IPR027050 - Nuclease SbcCD subunit C | 3'UTR_intron|outside_mRNA | |
| Cre13.g603000 | PDE19 | (1 of 17) 3.1.4.53 - 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGCAGGCCCCACAATTTGCTCTTCATC |
| Internal bar code: | TGACGACTATTTGCCTGATGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 99.9026 |
| LEAP-Seq n confirming: | 1026 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTGGTGGGCAGGTATT |
| Suggested primer 2: | ATTGACGCCAAAACTCCAAG |