| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.147143 |
| Chromosome: | chromosome 1 |
| Location: | 2160990 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011800 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAGCTCCCACTACAACTACTACACGAC |
| Internal bar code: | TCTAGCAGGACAGCAACTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 99.8447 |
| LEAP-Seq n confirming: | 7071 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAAACAACAGCCACACA |
| Suggested primer 2: | TACAGGTGAGGCAGAGGCTT |