| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.147180 |
| Chromosome: | chromosome 16 |
| Location: | 3724465 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688950 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGACCGCGTCCGCGGGCCTGCTGCTGCG |
| Internal bar code: | ATAAAGGAGGGCTGTCGCACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 99.7758 |
| LEAP-Seq n confirming: | 1335 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGCCGTACATCCCAGTA |
| Suggested primer 2: | AATGCCAAACGTCCTACCTG |