| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.147240 |
| Chromosome: | chromosome 4 |
| Location: | 127286 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216774 | (1 of 2) IPR000601 - PKD domain | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTCAACCCCCTGTTTATGCTCCCCTGA |
| Internal bar code: | AACCGTGTTGCTGCGTAACTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 194 |
| LEAP-Seq percent confirming: | 99.732 |
| LEAP-Seq n confirming: | 3722 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGCTCACCTGGAAT |
| Suggested primer 2: | AATATTTTGTTTGCGGCCTG |