| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147247 |
| Chromosome: | chromosome 8 |
| Location: | 1188418 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363100 | (1 of 2) PTHR23301//PTHR23301:SF0 - CHITIN BINDING PERITROPHIN-A // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAGCCTTTAAGTTTTGTTGGCGTTGCG |
| Internal bar code: | AGCGACGTCAGGCGGCAGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 767 |
| LEAP-Seq percent confirming: | 99.8867 |
| LEAP-Seq n confirming: | 882 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGCTGTGAATGCGGTAA |
| Suggested primer 2: | CGGCAAATGGAGGTAACACT |