| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.147271 |
| Chromosome: | chromosome 17 |
| Location: | 3565857 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725550 | GLD1 | Glucose-6-phosphate-1-dehydrogenase; (1 of 2) 1.1.1.49 - Glucose-6-phosphate dehydrogenase (NADP(+)) / GPD | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGACTGGTGTGTGGCCGTACGGACGCGCG |
| Internal bar code: | GAGGGTGGTAGAGATGACGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 201 |
| LEAP-Seq percent confirming: | 99.8239 |
| LEAP-Seq n confirming: | 2268 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATAACTCCTCGTCCCCCT |
| Suggested primer 2: | CTTCCTCCTCCATTTCCTCC |