| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147279 |
| Chromosome: | chromosome 14 |
| Location: | 777263 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612950 | CYG17 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCGTGCCGACGTGGCACGCACGCGCC |
| Internal bar code: | ACCAAACACCCTTCTTGGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 97.4684 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTTCTAGTGCTTGTTCG |
| Suggested primer 2: | TAGATGTCCAGCAGCGTGTC |