| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147283 |
| Chromosome: | chromosome_6 |
| Location: | 7021857 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g296850 | FAP81 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGTCGGGCTTGATCTTGAGCTCCGCCAGC |
| Internal bar code: | TGTGCCAAGGCTGTCAAACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 237 |
| LEAP-Seq percent confirming: | 90.0259 |
| LEAP-Seq n confirming: | 695 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATGGGCGTGTACTTTGA |
| Suggested primer 2: | GTGCAAGAGGAGGAGGAGG |