Insertion junction: LMJ.RY0402.147289_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.147289
Chromosome:chromosome 16
Location:139508
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g695218 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGTGCCCGGCGGCAACCAACTAGGGAAG
Internal bar code:TTGGGCATCTGGGCAAGCTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:510
LEAP-Seq percent confirming:99.8951
LEAP-Seq n confirming:4762
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTACATCCTGTAAGGCGT
Suggested primer 2:CATACACACACATGCGTCCA