| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147295 |
| Chromosome: | chromosome 14 |
| Location: | 876264 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613450 | CGL87 | (1 of 1) PTHR12818:SF0 - NEF-ASSOCIATED PROTEIN 1; Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGACGTGGTTGCAGGCATAGCGTTTC |
| Internal bar code: | TGAGGGTCGACCAGCTCGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 99.2019 |
| LEAP-Seq n confirming: | 3853 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAAGGGGAGAAAAGGAGG |
| Suggested primer 2: | ATTTGAGAAGGCAAGCGAAA |