Insertion junction: LMJ.RY0402.147321_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.147321
Chromosome:chromosome 9
Location:7527774
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g414150 GT90F42,GT90-42 GT90 family protein 42; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTCACTAGCGCTTTCACTTTCACCTTCA
Internal bar code:CGGGGGAGCTGGCGCAAGTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:751
LEAP-Seq percent confirming:99.8282
LEAP-Seq n confirming:1162
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGAGGTGTGGGTACAAGC
Suggested primer 2:CTTGGAACGGTTTGTTCGTT