| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.147333 |
| Chromosome: | chromosome 2 |
| Location: | 9164811 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143507 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGCGTCGGAGTGCGATAGCGGGAGCCA |
| Internal bar code: | GTGAGTGCCACTGCCCTTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 374 |
| LEAP-Seq percent confirming: | 99.2417 |
| LEAP-Seq n confirming: | 5235 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGACCACTAAGCACGCC |
| Suggested primer 2: | GTGTGTGTGGGTGTGTGTCA |