Insertion junction: LMJ.RY0402.147358_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.147358
Chromosome:chromosome 10
Location:4380796
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g451400 NIT4,CNX1B,CNX1E (1 of 1) 2.10.1.1//2.7.7.75 - Molybdopterin molybdotransferase // Molybdopterin adenylyltransferase; Molybdenum cofactor biosynthesis protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCTAGTAGGTCCCTGCCACTTTTTGGGC
Internal bar code:GGTCGCCAGGCCCTGGAAAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:721
LEAP-Seq percent confirming:99.6332
LEAP-Seq n confirming:1358
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGGCTGTGTATGGGTTG
Suggested primer 2:GAGGCTATGCGTGAGAAAGG