Insertion junction: LMJ.RY0402.147399_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.147399
Chromosome:chromosome 12
Location:1308388
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g488850 AP2A1 Alpha2-Adaptin; (1 of 1) K11824 - AP-2 complex subunit alpha (AP2A) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGCACCTTAAAACCCAGCCCAACACCA
Internal bar code:CTCACAACGGATCCACATCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:168
LEAP-Seq percent confirming:97.102
LEAP-Seq n confirming:2580
LEAP-Seq n nonconfirming:77
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCACGCAAACATATCGTA
Suggested primer 2:CAGCGTTTAGAGGTAAGGCG