| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.147399 |
| Chromosome: | chromosome_12 |
| Location: | 1308388 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g488850 | AP2A1 | Alpha2-Adaptin | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGCGCACCTTAAAACCCAGCCCAACACCA |
| Internal bar code: | CTCACAACGGATCCACATCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 168 |
| LEAP-Seq percent confirming: | 97.102 |
| LEAP-Seq n confirming: | 2580 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCACGCAAACATATCGTA |
| Suggested primer 2: | CAGCGTTTAGAGGTAAGGCG |