| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147402 |
| Chromosome: | chromosome 16 |
| Location: | 3014001 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664801 | RBL4 | rhomboid-like protease; (1 of 4) PTHR22936//PTHR22936:SF32 - RHOMBOID-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTAGGGTATGGGCTGTCCCATCACACAC |
| Internal bar code: | TCGAGGGTTCACTACTCGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1371 |
| LEAP-Seq percent confirming: | 99.3318 |
| LEAP-Seq n confirming: | 892 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGTGAATTCCCTATCTCG |
| Suggested primer 2: | ATCGCTTAGCTTGCTGGTGT |