| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147420 |
| Chromosome: | chromosome 6 |
| Location: | 5930221 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288600 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAAACCGCTCACCTTTTCAGGTCCTCCG |
| Internal bar code: | GCGTGATTGGCTCGCGGTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 922 |
| LEAP-Seq percent confirming: | 98.0705 |
| LEAP-Seq n confirming: | 1474 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAATCATTGTGTACGTGG |
| Suggested primer 2: | CAGCTATGGCTGGATGGTTT |