Insertion junction: LMJ.RY0402.147445_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.147445
Chromosome:chromosome 4
Location:2872920
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g224400 (1 of 1) 3.6.3.25//3.6.3.44 - Sulfate-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCTTGGCCATGTGTTCTCAAAGGCACCA
Internal bar code:ATACCGCTCTCAGTGCGCCTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:563
LEAP-Seq percent confirming:99.8765
LEAP-Seq n confirming:809
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGCAGGACGAGAGGAAA
Suggested primer 2:CCAGAGCTTGCTAGTGGTCC