| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147452 |
| Chromosome: | chromosome 8 |
| Location: | 3968011 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378800 | (1 of 1) PTHR10071:SF159 - GATA TRANSCRIPTION FACTOR 26-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGAGGCGCAGTACCTAGACGCGAGCT |
| Internal bar code: | TCTTAGGGACGGTCGTCTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 845 |
| LEAP-Seq percent confirming: | 99.8153 |
| LEAP-Seq n confirming: | 1081 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACTCGCTGGGTACTTC |
| Suggested primer 2: | TGTTTCAGTTCTGCGGTCAG |