| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.147453 |
| Chromosome: | chromosome 9 |
| Location: | 1906333 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395300 | (1 of 1) PTHR11736//PTHR11736:SF14 - MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN // MAGE PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGACGAGACGGCCGGGTAGGCGCACA |
| Internal bar code: | GGTACGTCAAGGACCCGTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 99.7678 |
| LEAP-Seq n confirming: | 5156 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCGCTGGGTATCTTGGA |
| Suggested primer 2: | ACCTGCTCACATATACCGCC |