| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147457 |
| Chromosome: | chromosome 7 |
| Location: | 5099553 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347800 | SMM29 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PTHR14614:SF30 - S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCTTGCCGGTCCCAGTGGGCGTTTCCA |
| Internal bar code: | AGGCGAGTGTTAATGGCGCTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 513 |
| LEAP-Seq percent confirming: | 95.5801 |
| LEAP-Seq n confirming: | 3287 |
| LEAP-Seq n nonconfirming: | 152 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTACCAAGGGCCTAGAAG |
| Suggested primer 2: | TGAAGAGAAGATGTGTGGCG |