| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147462 |
| Chromosome: | chromosome 8 |
| Location: | 1338 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTAATGTACACCACTCCCTAACTCCCT |
| Internal bar code: | GGCAGCTTTACCTGTAGAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 91.411 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTGCGTTAAGAAGCTGG |
| Suggested primer 2: | AACAGGATCCGTACCACTGC |