| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147464 |
| Chromosome: | chromosome 4 |
| Location: | 1967805 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218500 | (1 of 1) PTHR11732//PTHR11732:SF34 - ALDO/KETO REDUCTASE // 1,5-ANHYDRO-D-FRUCTOSE REDUCTASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGAGGAGGAGGGGGAGGGAAGAGGAG |
| Internal bar code: | CGTGCAGTGGCATGGGGTCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 98.8571 |
| LEAP-Seq n confirming: | 173 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTTCCGCAAGATGGTCT |
| Suggested primer 2: | ACCCACTTCACCTTCCACAG |