| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147481 |
| Chromosome: | chromosome 1 |
| Location: | 4829495 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033500 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTACGACATTGGCGTGGCGGTGGAGGA |
| Internal bar code: | TTGGGTTGTCTCCCAGAATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 97.8408 |
| LEAP-Seq n confirming: | 1450 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAAGTTACGGGCCTGTGT |
| Suggested primer 2: | TGGTTTGCTTTAGTTTGGGC |