| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147499 |
| Chromosome: | chromosome 2 |
| Location: | 2117749 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g089237 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGCCGAAGCACATGCCACATACTGTA |
| Internal bar code: | TTGCTTGTAATAGTTTGACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 140 |
| LEAP-Seq percent confirming: | 28.1102 |
| LEAP-Seq n confirming: | 714 |
| LEAP-Seq n nonconfirming: | 1826 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCACACGTTAACAATGCC |
| Suggested primer 2: | CTCCTGTCTGAACCCGTCTC |