| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147525 |
| Chromosome: | chromosome 9 |
| Location: | 6606933 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408464 | (1 of 1) PF10637 - Oxoglutarate and iron-dependent oxygenase degradation C-term (Ofd1_CTDD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGTAACAAGGCATGTGCCATGTGTGGAC |
| Internal bar code: | CAGTCACAACCTTATTTCATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 99.4796 |
| LEAP-Seq n confirming: | 2485 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACCATGGCCTTCTTCAC |
| Suggested primer 2: | CAGCCCCAAACTAAACCGTA |