Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.147528 |
Chromosome: | chromosome 3 |
Location: | 7177202 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g208100 | RNB1,Rrp44 | 3'-5' exoribonuclease II; (1 of 1) K18758 - DIS3-like exonuclease 2 [EC:3.1.13.-] (DIS3L2) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTTCTGAACATGGGACGTTACACGTGG |
Internal bar code: | CGCGCGTGCGCTGCAGAGGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 538 |
LEAP-Seq percent confirming: | 99.9041 |
LEAP-Seq n confirming: | 1042 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTGAGCCTGTTCTCTGACC |
Suggested primer 2: | CACATCTGGCCCGTTTAGTT |