| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147592 |
| Chromosome: | chromosome 17 |
| Location: | 1968673 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGAGGAAGGGACATGGTAGGGGTTAGCA |
| Internal bar code: | ATGGTGCGCGTCTACGGAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 98.1221 |
| LEAP-Seq n confirming: | 209 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGCACTGCGGAAGAATG |
| Suggested primer 2: | TATCGTGTTCAACAAAGCGG |