| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.147602 |
| Chromosome: | chromosome 1 |
| Location: | 7207419 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051800 | AAT,AST2 | (1 of 1) PTHR11751//PTHR11751:SF184 - SUBGROUP I AMINOTRANSFERASE RELATED // SUBFAMILY NOT NAMED; Aspartate aminotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGACATCTGTGGGTTTGGCCGCTTTGGC |
| Internal bar code: | ACTAATGTCCTACGGGGGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 68 |
| LEAP-Seq percent confirming: | 99.5984 |
| LEAP-Seq n confirming: | 248 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACATCCGCTGCTCCTAC |
| Suggested primer 2: | CGTCTGCAAGCATAAGACCA |