| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147606 |
| Chromosome: | chromosome 10 |
| Location: | 2040979 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432900 | AKR3 | Aldo/keto reductase; (1 of 1) PTHR11732//PTHR11732:SF170 - ALDO/KETO REDUCTASE // ALDOSE REDUCTASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTGGCACCCGTCGTGGCTCATGGTTGT |
| Internal bar code: | GAAAGTGTAATGGCCCTCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 99.6532 |
| LEAP-Seq n confirming: | 4597 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCAACATACTCGCTTCAC |
| Suggested primer 2: | ATCACTGGTTACTCACCGCC |