| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147607 |
| Chromosome: | chromosome 14 |
| Location: | 2615282 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625950 | PAP4 | (1 of 1) 2.7.7.19//2.7.7.72 - Polynucleotide adenylyltransferase / RNA adenylating enzyme // CCA tRNA nucleotidyltransferase / tRNA-nucleotidyltransferase; Class-II RNA nucleotidyl transferase 4, mitochondrial | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTCTCTCCATCGCCTCGGTTTGGAGAG |
| Internal bar code: | CGATTGCTTTGACTGTCGTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 93 |
| LEAP-Seq percent confirming: | 99.8674 |
| LEAP-Seq n confirming: | 753 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGCAGGGTTAGGGTAAG |
| Suggested primer 2: | AAACTAAAACGAGCGGAGCA |