| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147654 |
| Chromosome: | chromosome 10 |
| Location: | 1430597 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428400 | PUS7 | (1 of 2) 5.4.99.24 - 23S rRNA pseudouridine(955/2504/2580) synthase; RNA pseudouridine synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCGGGCTGGCCGCTACCGAGGGACGG |
| Internal bar code: | AGTGGCTATGGTCCGGGCGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 886 |
| LEAP-Seq percent confirming: | 99.2209 |
| LEAP-Seq n confirming: | 5731 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACACACAACGCATCGCAT |
| Suggested primer 2: | TTATGGAAGTGCGAGTGCTG |