| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.147668 |
| Chromosome: | chromosome 15 |
| Location: | 524541 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g637000 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCAGCTACAGCTGCTACAGCCGGTCTC |
| Internal bar code: | TTCTTGGTTTCCTCTTGGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 99.6727 |
| LEAP-Seq n confirming: | 4263 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGCTGGTGGTACTGGT |
| Suggested primer 2: | CTCTCCTGTCCTTCTGTGCC |