| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.147677 |
| Chromosome: | chromosome 7 |
| Location: | 2424688 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328850 | CNK8 | NimA-related protein kinase; (1 of 1) PTHR24361:SF240 - PROTEIN MTK-1, ISOFORM B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCGCTAACCTCCCTTCCTCCCTTCCCA |
| Internal bar code: | AGCATGAAGGTCGTAGTTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 98.5611 |
| LEAP-Seq n confirming: | 274 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTCTTCAAGCACACGGA |
| Suggested primer 2: | CATGACTGCGAGTGTGGTTT |