Insertion junction: LMJ.RY0402.147690_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.147690
Chromosome:chromosome 16
Location:2579831
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g661500 (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAATCCTCCTTCACAAAGCACGGCCCGCA
Internal bar code:AATCTATGGCGTTGTCCCTAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:202
LEAP-Seq percent confirming:99.0832
LEAP-Seq n confirming:1405
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AATAAAAGGCGTCGTGGTTG
Suggested primer 2:GTAGCATTGCAGCCTTGTCA