| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.147691 |
| Chromosome: | chromosome 1 |
| Location: | 385617 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002400 | TGL1 | (1 of 1) 3.1.1.3//3.1.1.73 - Triacylglycerol lipase / Triglyceride lipase // Feruloyl esterase / Hydroxycinnamoyl esterase; Putative triacylglycerol lipase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGCTAAAATACTGTTTCTTAACTAATC |
| Internal bar code: | AGAGTATCCGACCGCCGGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 9 |
| LEAP-Seq percent confirming: | 60.3175 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTCTGCTTCATCGACCA |
| Suggested primer 2: | GTGGATAAAGGTGGAGGGGT |