| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147734 |
| Chromosome: | scaffold 40 |
| Location: | 1158 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre40.g760147 | (1 of 1) PTHR10877//PTHR10891 - POLYCYSTIN-RELATED // EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGAATGGTGAAGCAGCCCCTGCACCAC |
| Internal bar code: | ATGTGTGACATAAAGGTAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1114 |
| LEAP-Seq percent confirming: | 99.5609 |
| LEAP-Seq n confirming: | 6802 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCGAGGTAAGTGTTGTA |
| Suggested primer 2: | GAACAGCAAGGTGTCAAGCA |