Insertion junction: LMJ.RY0402.147747_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.147747
Chromosome:chromosome 10
Location:392357
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g420550 TSP4,CTPA4,CTPA1 (1 of 1) IPR004447 - C-terminal-processing peptidase S41A; C-terminal peptidase A 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGGGCCTGAGGGTGGGGACTTGCTAAAA
Internal bar code:CTGAGGTGTCGACGGAGGACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:579
LEAP-Seq percent confirming:99.6819
LEAP-Seq n confirming:940
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GACAAGTCCCTCAACCTGGA
Suggested primer 2:AAAGATGGACGATGCGAAAC