| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147751 |
| Chromosome: | chromosome 3 |
| Location: | 3722164 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169550 | AOX2 | Alternative oxidase; (1 of 2) PTHR31803:SF3 - ALTERNATIVE OXIDASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGCGTTGCCCCCCCCTCTCGGGGGCTC |
| Internal bar code: | CCTGGCATATTAAATGGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 99.7409 |
| LEAP-Seq n confirming: | 5005 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACCTGGCTAAAAACCAAA |
| Suggested primer 2: | CATGGCGTACGATGTACCTG |