| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.147787 |
| Chromosome: | chromosome 14 |
| Location: | 961282 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613900 | CYCT1 | (1 of 2) PTHR10026//PTHR10026:SF51 - CYCLIN // CYCLIN-K; T-type cyclin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCAAGGGTCCTCAGCGCTCAGCCTCACT |
| Internal bar code: | AAGAGACACAGCACGCGGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 98.8489 |
| LEAP-Seq n confirming: | 6269 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGTCCCAAGATTGCTGT |
| Suggested primer 2: | AAGAAGAGCACTGGCAGCTC |