| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.147790 |
| Chromosome: | chromosome_3 |
| Location: | 1617657 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g152750 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACATGCCTTACTGACTTGACAGTTTGCAGT |
| Internal bar code: | CACCTGTCTATCCATCGTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 99.8686 |
| LEAP-Seq n confirming: | 1520 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCCCGTAACAGCCTATG |
| Suggested primer 2: | GTGGTGCTGTAAGCCTGTCA |