| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147801 |
| Chromosome: | chromosome 16 |
| Location: | 404660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693450 | ISG-C1 | (1 of 32) IPR029062 - Class I glutamine amidotransferase-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCGAGGGGTAGGCGCCGTACGGGCCGT |
| Internal bar code: | CGGATGTCACGGGTTTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 99.1071 |
| LEAP-Seq n confirming: | 111 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCTCATCTCCTGCAACC |
| Suggested primer 2: | GACTGTGTGGGGTAGCCACT |