Insertion junction: LMJ.RY0402.147805_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.147805
Chromosome:chromosome 7
Location:2814879
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g331750 (1 of 7) PF12680 - SnoaL-like domain (SnoaL_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGGTTGCGTTTGAGGCTTGTGCGTGTG
Internal bar code:TGCGCTCGCGGGATCGGACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:864
LEAP-Seq percent confirming:99.7243
LEAP-Seq n confirming:4340
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTACTAAGGCCAACTTGC
Suggested primer 2:GGCAGGACATGGAAACTTGT