| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.147806 |
| Chromosome: | chromosome 10 |
| Location: | 5996434 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462750 | (1 of 1) K11374 - elongator complex protein 2 (ELP2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGAAAGAGCGAGGACGGGGGTGGGGGT |
| Internal bar code: | TCCACCTGGTTTGGTGGAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 96.8421 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTCGTACACAGCCTTC |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |